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bioRxiv 预印本· Migliori, V., Suo, C., Dufva, O., Gyulev, I., Polanski, K., Symeonidou, V., Rodschinka, G., Sarropoulos, I., Iwama, S., Steemers, A. S., Gontarczyk, A. M., Powell, H., Cujba, A.-M., Park, J.-E. S., Garnett, M., Vassiliou, G. S., Teichmann, S. A., Bassett, A. R. ·· 17 小时前AI 评分22

STEER-seq:基于重组酶的池化多模态单细胞扰动筛选平台

STEER-seq: a recombinase based platform for pooled multimodal single-cell perturbation screening

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研究人员开发了STEER-seq(Single-cell Transgene Expression via Exchange Recombination sequencing),一种无需病毒的池化ORF功能获得筛选平台,通过Bxb1介导的基因组安全港位点整合与无启动子供体文库,实现单拷贝表达和单细胞测序直接读取扰动身份。

来源:bioRxiv 预印本 · biorxiv.org